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報(bào)告題目：細(xì)胞壁多糖合成的酶學(xué)探究

報(bào)告人：曾為（墨爾本大學(xué)）

時(shí)間：2016年9月22日（周四）上午9:00

地點(diǎn)：智能實(shí)驗(yàn)樓學(xué)術(shù)報(bào)告廳

報(bào)告人簡(jiǎn)介：

曾為，湖南湘潭人。2001年本科畢業(yè)于北京師范大學(xué)生命科學(xué)學(xué)院。2009年博士畢業(yè)于美國(guó)俄亥俄大學(xué)（Ohio University)細(xì)胞和分子生物學(xué)專(zhuān)業(yè)?，F(xiàn)在澳大利亞墨爾本大學(xué)（University of Melbourne)植物細(xì)胞壁研究中心（ARC Centre of Excellence in Plant Cell Walls) Tony Bacic研究組工作。植物細(xì)胞壁多糖由糖基轉(zhuǎn)移酶（Glycosyltransferase）參與合成，但其合成分子機(jī)理尚不清楚。其研究?jī)?nèi)容主要是運(yùn)用分子生物學(xué)和生物化學(xué)分析植物糖基轉(zhuǎn)移酶的功能，使用外源系統(tǒng)表達(dá)植物糖基轉(zhuǎn)移酶并設(shè)計(jì)酶學(xué)反應(yīng)，在試管中模擬植物多糖的合成，并用高效液相色譜（HPLC），質(zhì)譜（Mass Spectrometry），核磁共振（NMR）等技術(shù)手段對(duì)產(chǎn)物進(jìn)行鑒定。已在木聚糖(Xylan) 合成研究中取得一定突破，探索了植物細(xì)胞壁合成機(jī)理，對(duì)進(jìn)一步改造植物細(xì)胞壁，讓其更好服務(wù)人類(lèi)提供了理論基礎(chǔ)。

Publications:

1. Zeng, W.; Chatterjee, M.; Faik, A. Udp-xylose-stimulated glucuronyltransferase activity in wheat microsomal membranes: Characterization and role in glucurono(arabino)xylan biosynthesis. Plant physiology 2008, 147, 78-91.

2. Zeng, W.; Jiang, N.; Nadella, R.; Killen, T.L.; Nadella, V.; Faik, A. A glucurono(arabino)xylan synthase complex from wheat contains members of the GT43, GT47, and GT75 families and functions cooperatively. Plant physiology 2010, 154, 78-97.

3. Li, L.; Huang, J.; Qin, L.; Huang, Y.; Zeng, W.; Rao, Y.; Li, J.; Li, X.; Xu, W. Two cotton fiber-associated glycosyltransferases, GhGT43a1 and GhGT43c1, function in hemicellulose glucuronoxylan biosynthesis during plant development. Physiologia plantarum 2014, 152, 367-379.

4. Zhao, X.; Ouyang, K.; Gan, S.; Zeng, W.; Song, L.; Zhao, S.; Li, J.; Doblin, M.S.; Bacic, A.; Chen, X.Y., et al. Biochemical and molecular changes associated with heteroxylan biosynthesis in neolamarckia cadamba (rubiaceae) during xylogenesis. Frontiers in plant science 2014, 5, 602.

5. Ford, K.L.; Zeng, W.; Heazlewood, J.L.; Bacic, A. Characterization of protein n-glycosylation by tandem mass spectrometry using complementary fragmentation techniques. Frontiers in plant science 2015, 6, 674.

6. Song, L.; Zeng, W.; Wu, A.; Picard, K.; Lampugnani, E.R.; Cheetamun, R.; Beahan, C.; Cassin, A.; Lonsdale, A.; Doblin, M.S., et al. Asparagus spears as a model to study heteroxylan biosynthesis during secondary wall development. PloS one 2015, 10, e0123878.

7. Kuang, B.; Zhao, X.; Zhou, C.; Zeng, W.; Ren, J.; Ebert, B.; Beahan, C.T.; Deng, X.; Zeng, Q.; Zhou, G., et al. The role of udp-glucuronic acid decarboxylase (UXS) in xylan biosynthesis in arabidopsis. Molecular plant 2016, 9, 1119-1131.

8. Zeng, W.; Lampugnani, E.R.; Picard, K.L.; Song, L.; Wu, A.M.; Farion, I.M.; Zhao, J.; Ford, K.; Doblin, M.S.; Bacic, A. Asparagus IRX9, IRX10, and IRX14A are components of an active xylan backbone synthase complex that forms in the Golgi apparatus. Plant physiology 2016, 171, 93-109.

9. Ford K.; Chin T.; Srivastava V.; Zeng W.; Doblin M.; Bulone V.; Bacic A. Comparative “Golgi” proteome study of Lolium multiflorum and Populus trichocarpa. Proteomes 2016, 4, 23.

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林業(yè)與生物技術(shù)學(xué)院

2016年9月21日